Nano-aAPC Preparation via MHC-Ig Conjugation

Soluble MHC-Ig dimers, K^b-Ig, was prepared and loaded with peptides as described (17 (link)), see Supplemental Materials. Nano-aAPC were manufactured by direct conjugation of MHC-Ig dimer and anti-CD28 antibody (37.51; Biolegend) to MACS Microbeads (Miltenyi Biotec) as described previously (18 (link)).

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Sidhom J.W., Bessell C.A., Havel J.J., Kosmides A., Chan T.A, & Schneck J.P. (2017). ImmunoMap: A Bioinformatics Tool for T-Cell Repertoire Analysis. Cancer immunology research, 6(2), 151-162.

Publication 2017

anti-CD28 antibody (37.51; MACS Microbeads

Aapc Anti antibody Microbeads Peptides

Corresponding Organization : Johns Hopkins Medicine

Other organizations : Sidney Kimmel Comprehensive Cancer Center, Memorial Sloan Kettering Cancer Center

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Variable analysis

independent variables

Preparation of soluble MHC-Ig dimers, K^b-Ig
Loading of peptides onto MHC-Ig dimers
Direct conjugation of MHC-Ig dimer and anti-CD28 antibody to MACS Microbeads

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive control: None mentioned
Negative control: None mentioned

Annotations

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