CS activity was used as a marker of mitochondrial content 35 (link),36 (link). CS was measured using a kit from Sigma aldrich (#MAK193) following the manufacturer’s instruction. Briefly, 10 mg of brain cortex was homogenized in 100 μl of ice-cold CS Assay Buffer. Then, the samples were centrifuged at 10,000 g for 5 minutes and the supernatant was transferred to a fresh tube. 25 μl of sample was added to wells of 96-well plates in duplicate with appropriate reaction mixes (CS assay buffer, developer and substrate mix). A standard-curve was obtained with serial dilutions of GSH solution (0 to 40 nmol/well). The plate was incubated for 3 minutes at 25°C and the absorbance was recorded at 412nm every 5 minutes for 30 minutes. The colorimetric product (GSH) was proportional to the enzymatic activity of CS and normalized to the quantity of tissue.