STZ-induced T1D mice were generated as previously described.9 (link) In brief, STZ (Cat #S0130, Sigma-Aldrich, St Louis, Missouri) was dissolved in 0.05 M sodium citrate buffer and intraperitoneally injected into male C57BL/6J mice (4–5 weeks old) at a dose of 55 mg/kg body weight (mpk) daily for 5 days. After STZ injection, these mice were housed in the pathogen-free vivarium for 14–48 days and blood glucose levels were measured using a glucometer as described in Lan et al.8 (link) Serum insulin levels in these mice at 48 days post-intraperitoneal STZ treatment were also determined using Thermo Fisher Scientific’s Mouse Insulin ELISA-Kit (Cat #EMINS), according to the manufacturer’s protocol.
Protocol detail
Streptozotocin-Induced Diabetic Mouse Model
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Blood glucose
Body weight
Buffer
C57bl mice
Elisa
Insulin
Intraperitoneal treatment
Male
Mouse
Pathogen
Serum
Sodium citrate
Corresponding Organization : Alltech (United States)
Other organizations : University of Louisville
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Variable analysis
independent variables
- Streptozotocin (STZ) dose (55 mg/kg body weight) administered intraperitoneally daily for 5 days
- Blood glucose levels measured at 14-48 days post-STZ injection
- Serum insulin levels at 48 days post-STZ injection
- Male C57BL/6J mice (4-5 weeks old)
- STZ dissolved in 0.05 M sodium citrate buffer
- Mice housed in pathogen-free vivarium
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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