Quantitative Immunoblotting for Protein Analysis

Protein levels from rat kidney cortex homogenates and from cell lysates were quantitated using immunoblotting as described by us previously73 (link)74 (link)75 (link)76 (link)77 (link)78 (link)79 (link)80 (link). The antibodies against HDACs (Cell signalling technology, MA USA), E-cadherin, Fibronectin, Collagen, RAGE (Santa Cruz Biotechnology, USA), TGF-beta, Histone H3, acetyl Histone H3 (Abcam MA USA) and anti-acetyl Histone H4 (Millipore) and, anti-β-actin (Cell signalling technology, MA USA) were used and signals were detected using a chemi-luminiscence-based detection system (Amersham). The levels of protein expression were quatitated using the Quantity one (BioRad) software.

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Singh R.S., Chaudhary D.K., Mohan A., Kumar P., Chaturvedi C.P., Ecelbarger C.M., Godbole M.M, & Tiwari S. (2016). Greater efficacy of atorvastatin versus a non-statin lipid-lowering agent against renal injury: potential role as a histone deacetylase inhibitor. Scientific Reports, 6, 38034.

Publication 2016

E-cadherin Fibronectin Histone H3 acetyl Histone H3 anti-acetyl Histone H4 anti-β-actin Quantity one

Actin Antibodies Cell Collagen E cadherin Fibronectin Histone h3 Histone h4 Kidney cortex Protein Rage Tgf beta

Corresponding Organization :

Other organizations : Sanjay Gandhi Post Graduate Institute of Medical Sciences, Georgetown University

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Variable analysis

independent variables

Protein levels from rat kidney cortex homogenates
Protein levels from cell lysates

dependent variables

Protein expression levels

control variables

Antibodies against HDACs, E-cadherin, Fibronectin, Collagen, RAGE, TGF-beta, Histone H3, acetyl Histone H3, and anti-acetyl Histone H4
Anti-β-actin antibody

controls

Positive controls not explicitly mentioned
Negative controls not explicitly mentioned

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