Collagen-Induced Arthritis in DBA/1 Mice

Collagen-induced arthritis was performed as referred in Kim et al. [21 (link)]. At day 0, DBA/1 male mice (age 6 weeks), obtained from Janvier (Le Genest Saint Isle) were injected subcutaneously at the tail base with 100 μg of bovine CII (Chondrex) emulsified in complete Freund’s adjuvant (CFA). A booster injection of 100 μg of CII in incomplete Freund’s adjuvant (IFA) was performed subcutaneously at day 21. All animal experiments were conducted in accordance with institutional guidelines. The agreement numbers of the animal facilities are B76-450-05, B76-540-7 and experimentator is A.76-91. This study has been approved by the ethics committee (Comité Regional d’éthique en Expérimentation Animale Normandie) with the following numbers N/02-12-10/24/12-13.