SERS-Based Antibiotic Detection Protocol

Antibiotic detection was performed using SERS (ONSPEC, SCI Innovatech Co., Ltd., Thailand) using silver nanorods as the substrate. The substrate was prepared using a direct current magnetron sputtering system combined with a glancing-angle deposition technique [22 ]. Raman measurements were performed using a handheld Raman spectrometer (Mira M-3, Metrohm AG, Switzerland) with a 785-nm excitation laser. The laser power was 5 mW for measurements in both the solutions and on SERS substrate. The accumulation time for Raman measurements from the solution and on SERS substrate was 20 and 5 s, respectively. Three standard antibiotics (enrofloxacin [100 mg/mL; Bezter, Enro tec 500, Betagro Public Co., Ltd., Thailand], oxytetracycline [50 mg/mL; Oxyclin, General Drugs House Co., Ltd., Thailand], and neomycin [500 g/kg; Neomycin-500, Neotech Impex Co., Ltd., Thailand]) were procured from a veterinary pharmacy and used without further purification.

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Wattanavichean N., Nimittrakoolchai O.U., Nuntawong N., Horprathum M., Eiamchai P., Limwichean S., Somboonsaksri P., Sreta D, & Meesuwan S. (2023). A novel portable Raman scattering platform for antibiotic screening in pig urine. Veterinary World, 16(1), 204-214.

Publication 2023

Antibiotic Antibiotics Enrofloxacin Neomycin Oxytetracycline Pharmacy veterinary Silver

Corresponding Organization :

Other organizations : Mahidol University, Advanced Neural Dynamics (United States), National Science and Technology Development Agency, National Electronics and Computer Technology Center, Rajamangala University of Technology Tawan-ok

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Variable analysis

independent variables

Preparation of silver nanorods substrate using direct current magnetron sputtering system combined with a glancing-angle deposition technique

dependent variables

Antibiotic detection using SERS (surface-enhanced Raman spectroscopy)
Raman measurements performed using a handheld Raman spectrometer with a 785-nm excitation laser

control variables

Laser power of 5 mW for measurements in both the solutions and on SERS substrate
Accumulation time for Raman measurements from the solution and on SERS substrate (20 s and 5 s, respectively)

controls

Three standard antibiotics (enrofloxacin, oxytetracycline, and neomycin) were used without further purification

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