Venoms from African Snake Species

Venoms from Bitis arietans (Puff adder), Naja haje (Egyptian Cobra), and Naja pallida (Red spitting Cobra) were collected from wild-caught snakes at the East African Venom Supplies (Kenya). They were lyophilized and stored at −4°C at the Pharmacology and Toxicology Lab, Faculty of Veterinary Medicine, University of Nairobi. Each of the lyophilized venoms were accurately weighed (0.25 g) in an analytical balance and triturated using a pestle and mortar. The triturated venoms were transferred to 10 mL volumetric flasks and made up to the mark with phosphate buffered saline to make a 25 mg/mL concentration.