SARS-CoV-2 NSP10 and NSP14 Cloning

pDONR223 SARS-CoV-2 NSP10 (Addgene; plasmid no. 141264; http://n2t.net/addgene:141264; Research Resource Identifier: Addgene_141264) and pDONR223 SARS-CoV-2 NSP14 (Addgene; plasmid no. 141267; http://n2t.net/addgene:1412647; Research Resource Identifier: Addgene_141267); both were a kind gift from Fritz Roth (34 (link)). Using Gateway LR technology (Invitrogen), NSP10 and NSP14 were subcloned individually into gateway competent expression vector pDEST527 (N-terminal His-tags NSP10 and NSP14) for bacterial expression.

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Riccio A.A., Sullivan E.D, & Copeland W.C. (2021). Activation of the SARS-CoV-2 NSP14 3′–5′ exoribonuclease by NSP10 and response to antiviral inhibitors. The Journal of Biological Chemistry, 298(1), 101518.

Publication 2021

pDONR223 SARS-CoV-2 NSP14 Gateway LR technology

Bacterial Plasmid Sars cov 2 Vector

Corresponding Organization :

Other organizations : National Institute of Environmental Health Sciences

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Variable analysis

independent variables

PDONR223 SARS-CoV-2 NSP10
PDONR223 SARS-CoV-2 NSP14

dependent variables

Bacterial expression of NSP10 and NSP14 proteins with N-terminal His-tags

control variables

Gateway LR technology (Invitrogen)
PDEST527 expression vector

controls

Positive control: None mentioned
Negative control: None mentioned

Annotations

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