LIN28A-miR205HG Interaction Analysis

To estimate physical interaction between LIN28A protein and miR205HG, a cross‐linking immunoprecipitation (CLIP) assay was conducted as previously reported protocol [21 (link)]. Briefly, TE6 and OE21 cells (1 × 10⁷) were washed using 1× PBS and UV cross‐linked at a dose of 400 mJ·cm⁻². Then, cells were lysed using mild RIPA Lysis Buffer (Cat: PH0317, Wuhan, China) containing protease inhibitor (Cat. HY‐K0010; MedChemExpress, Shanghai, China) and RNase inhibitor (Cat: AM2694; Thermo Fisher). After precleaning with protein G sepharose beads (Cat: P3296; Sigma, Darmstadt, Germany), cell lysates were incubated with LIN28A antibody (1 μg) at 4 °C for 3 h. Next, the solution containing antibody–RNA complexes was incubated with BAS‐blocked protein G sepharose beads at 4 °C for 3 h. After being washed with washing buffer containing protease and RNase inhibitor, the beads bound with RNP complex were eluted for the subsequent RNA isolation.