Knockdown of HDAC2, YY1 and p300 in cells

pENTER-HDAC2 and YY1 or p300 and empty vector (pENTER) plasmids encoding a FLAG or HA tag were purchased from Vigene (Rockville, MD, USA). Ablation of HDAC2, YY1 or p300 was performed by transfection with siRNA duplex oligos, which were synthesised by Genepharma Company (Shanghai, China). The sequences of the siRNAs were as follows: (sense strand) Scrambled siRNA, HDAC2 siRNA: 5’-AAGCATCAGGATTCTGTTA-3ʹ, YY1 siRNA: 5ʹ-CGAGGATCAGATTCTCATC-3ʹ^{41 (link)}, and p300 siRNA: 5ʹ-AACCCCUCCUCUUCAGCACCA-3ʹ. Cell transfection was performed with Lipofectamine TM 2000 (Invitrogen) as described in the manufacturer’s protocol.