Histological Analysis of Spinal Cords

After anesthetization with excessive 20% urethane, the mice were perfused intracardially with phosphate buffered saline (PBS) followed by 4% paraformaldehyde. The dissected spinal cords were fixed in 4% paraformaldehyde, dehydrated in 15% and 30% sucrose PBS solution, and cut into 20 μm coronal sections. Thereafter, the sections were subjected to haematoxylin and eosin (H&E) staining and luxol fast blue (LFB) staining as described previously [27 (link),38 (link)]. The images were taken by Olympus VS120 Virtual Slide Scanner.

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Zhang W., Liu M., Yang L., Huang F., Lan Y., Li H., Wu H., Zhang B., Shi H, & Wu X. (2019). P-glycoprotein Inhibitor Tariquidar Potentiates Efficacy of Astragaloside IV in Experimental Autoimmune Encephalomyelitis Mice. Molecules, 24(3), 561.

Publication 2019

VS120 Virtual Slide Scanner

Eosin Haematoxylin Luxol fast blue Mice Paraformaldehyde Phosphate Saline solution Spinal cords Sucrose Urethane

Corresponding Organization : Shanghai University of Traditional Chinese Medicine

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Variable analysis

independent variables

Anesthetization with excessive 20% urethane

dependent variables

Histological appearance of spinal cord sections after H&E and LFB staining

control variables

Perfusion with phosphate buffered saline (PBS)
Fixation in 4% paraformaldehyde
Dehydration in 15% and 30% sucrose PBS solution
Cutting of sections into 20 μm coronal sections

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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