Circadian Rhythms and Neurogenesis Analysis

Sample sizes were determined according to previous studies [24 (link), 44 (link), 45 (link)]. All data were tested for normality using the Kolmogorov-Smirnov test prior to further statistical evaluation. Cosinor analysis was performed to determine circadian oscillations. Data were analyzed using CircWave statistic software (V1.4) according to the eq. Y = c + aSIN(i2π t/24) + bCOS(i2π t/24), where Y is delta cycle threshold, t is circadian time, and a, b, and c were predicted. Further nonlinear regression analysis was used to determine peak time, trough and amplitude of the curve, using the center of gravity parameter. Behavioral experiments were analyzed using 2-way ANOVA, repeated measure ANOVA and 3-way ANOVA. Neurogenesis and mitochondrial results were evaluated by Student’s t-test or corrected with Welch’s t-test, where appropriate. Electrophysiological data were analyzed using repeated measure ANOVA. Statistical outliers (values outside of the interval: mean ± 2 standard deviations) were excluded from further analyses. The threshold for significance was set at *p < 0.05 in all instances. Statistical analyses were performed using SPSS software for Windows, Version 24 (IBM Corporation, Chicago, USA) and Graphpad Prism, Version 7 (San Diego, USA).

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Berger S., Stattmann M., Cicvaric A., Monje F.J., Coiro P., Hotka M., Ricken G., Hainfellner J., Greber-Platzer S., Yasuda M., Desnick R.J, & Pollak D.D. (2020). Severe hydroxymethylbilane synthase deficiency causes depression-like behavior and mitochondrial dysfunction in a mouse model of homozygous dominant acute intermittent porphyria. Acta Neuropathologica Communications, 8, 38.

Publication 2020

SPSS software for Windows, Version 24

Anova Gravity Mitochondrial Neurogenesis Prism Student

Corresponding Organization :

Other organizations : Medical University of Vienna, Icahn School of Medicine at Mount Sinai

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Variable analysis

independent variables

Circadian time (t)

dependent variables

Delta cycle threshold (Y)

control variables

Sample sizes determined according to previous studies
Data tested for normality using Kolmogorov-Smirnov test
Statistical outliers (values outside of the interval: mean ± 2 standard deviations) excluded from further analyses

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