Platelet releasates were prepared as previously described53 (link) with washed platelets at ~1 × 108/mL activated by 0.5IU human α-thrombin (Hyphen Biomed, Neuville-sur-Oise, France) for 10 min. Platelet activation was assessed by expression of CD62P and releasates collected following centrifugation (2,800g for 10min) and stored at −20 °C until used.
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