MTT and BrdU Assays for Cell Viability

Cell viability was determined using a MTT metabolism test as described [44 (link)]. Briefly, at given times after treatment, MTT stock solution was added to a final concentration of 0.5 mg/mL and after 1 h formazan crystals were dissolved in DMSO. Optical densities were measured at 570 nm and 620 nm using a scanning multiwell spectrophotometer. Cell proliferation was assessed using ELISA BrdU kit (Roche Diagnostics GmbH, Germany) according to the manufacturer’s protocol. For MTT and BrdU assays, cells were seeded at a density of 4 × 10³ cells/well.

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Wojnicki K., Kaczmarczyk A., Wojtas B, & Kaminska B. (2023). BLM helicase overexpressed in human gliomas contributes to diverse responses of human glioma cells to chemotherapy. Cell Death Discovery, 9, 157.

Publication 2023

ELISA BrdU kit

After treatment Assays Brdu Cell proliferation Cell viability Cells Diagnostics Dmso Elisa Formazan Metabolism Optical

Corresponding Organization :

Other organizations : Instytut Biologii Doświadczalnej im. Marcelego Nenckiego

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Variable analysis

independent variables

Treatment

dependent variables

Cell viability (MTT metabolism test)
Cell proliferation (ELISA BrdU kit)

control variables

Cell density (4 × 10^3 cells/well)

controls

Positive control: Not mentioned
Negative control: Not mentioned

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