Osteogenic differentiation was achieved using mesenchymal stem cell osteogenic differentiation medium (PromoCell, Heidelberg, Germany). The medium was changed every 72 h; after 14 days, and cells were analyzed by Alizarin Red staining. Three photographs in 3 experiments were selected and run on the freely available imaging software ImageJ (version 1.52a, Stuttgart, Germany). Analysis was performed by threshold converting the 8-bit red–green–blue image into a binary image, consisting only of the pixels representing calcium deposits. The resulting percentage of the stained area was then compared to control images [50 (link)].
Giordani C., Matacchione G., Giuliani A., Valli D., Scarpa E.S., Antonelli A., Sabbatinelli J., Giacchetti G., Sabatelli S., Olivieri F, & Rippo M.R. (2023). Pro-Osteogenic and Anti-Inflammatory Synergistic Effect of Orthosilicic Acid, Vitamin K2, Curcumin, Polydatin and Quercetin Combination in Young and Senescent Bone Marrow-Derived Mesenchymal Stromal Cells. International Journal of Molecular Sciences, 24(10), 8820.
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to
get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
