Conditional Knockdown of Atp6ap2 in Mouse Kidney

The generation and genotype of ATP6ap2^flox/y has been already described [59 (link)]. Heterozygous ATP6ap2 ^flox/x females were crossed with males both positive for Pax8-rtTA and Cre [44 (link), 69 (link)]. Expression of Cre-recombinase was induced in male ATP6ap2^{flox/y,Pax8Cre+} and ATP6ap2^+/+,Pax8Cre+ transgenic mice by 1 mg/mL of doxycycline (Sigma) in drinking water containing 2% of Sucrose (Sigma) for 5 days followed by 5 days induction with 0.25 mg/mL doxycycline in 2% Sucrose drinking water and 4 days without doxycycline [44 (link)]. A second group of ATP6ap2^{flox/y,Pax8Cre+} and ATP6ap2^+/+,Pax8Cre+ transgenic mice received 2 mg/mL doxycycline for 5 days followed by 5 days of 1 mg/mL doxycycline in water containing 2% Sucrose [69 (link)]. These groups are accordingly referred to as 1 mg and 2 mg doxycycline. Animals used for experiments were 2- to 3-month-old male mice bred in a C57BL/6 background. All animal studies were performed according to Swiss welfare laws and with approval of the local veterinary authorities.