Characterization of Medulloblastoma Cell Lines

Two different and well characterized cell lines were used in our study, DAOY cells that directly derived from primary MB and ONS-76, described as a more immature cell line with a primitive profile [54 (link)]. DAOY and ONS-76 human MB cell lines were purchased from the American Type Culture Collection, (Manassas, VA). DAOY cells were cultured in Eagle's minimal essential medium (EMEM) supplemented with 1% Penicillin/Streptomycin and 10% fetal bovine serum (FBS) (all from Sigma-Aldrich, Milan, Italy) while ONS-76 was cultured in RPMI 1640 (GIBCO Cell Culture, Burlington, Ontario) containing 10% FBS, 1% L-glutamine 2 mM and 1% Penicillin/Streptomycin (Sigma-Aldrich). Cells were treated with 4-HPR (a kind gift from Dr. James A. Crowell, Division of Cancer Prevention, National Cancer Institute, Bethesda, MD, and Dr. Gregg Bullard, McKessonBio, Rockville, MD) dissolved in absolute ethanol in a stock solution of 10 mM and used at indicated concentrations for different time periods.