Regulation of Autophagy by Cholesterol Metabolism

Chloroquine was purchased from Wako Pure Chemical Industries (Osaka, Japan). 3-MA, rapamycin, and dynasore were purchased from Cayman Chemical (Ann Arbor, MI). A polyclonal anti-p62 antibody was purchased from Cell Signaling Technology, Inc. (Beverly, MA), a polyclonal anti-β-actin antibody was from Sigma (St. Louis, MO), and a polyclonal anti-LAMP2 antibody was from Bioss Antibodies (Woburn, MA). An inhibitor of cathepsin B (CA-074) was purchased from Peptide Institute, Inc. (Osaka, Japan). Nystatin and cytochalasin D were purchased from Sigma. A monoclonal anti-apoA-I antibody (Wt20-7) was produced as previously described14 (link). pEGFP-N1-TFEB was a gift from Shawn Ferguson (Addgene plasmid #38119)59 (link).