Human iPSC Culture Protocol

Human iPSCs (in the male WTC11 background (Miyaoka et al., 2014) were cultured in StemFlex Medium on BioLite Cell Culture Treated Dishes (Thermo Fisher Scientific; assorted Cat. No.) coated with Growth Factor Reduced, Phenol Red-Free, LDEV-Free Matrigel Basement Membrane Matrix (Corning; Cat. No. 356231) diluted 1:100 in Knockout DMEM (GIBCO/Thermo Fisher Scientific; Cat. No. 10829–018). Routine passaging was performed as described ^{23 (link)}. Studies with human iPSCs at UCSF were approved by the The Human Gamete, Embryo and Stem Cell Research (GESCR) Committee. Informed consent was obtained from the human subjects when the WTC11 (Miyaokaet al., 2014) lines were originally derived.