MoBY-ORF Collection Protocols

The MoBY-ORF collection will be available through Open Biosystems. The Supplementary Methods online lists the strains used in this study, growth media, plasmid pool preparation method, MoBY-ORF clone construction methods and analysis, procedures for sequencing of the MoBY-ORF collection, procedures for functional complementation analysis of essential genes with MoBY-ORF plasmids and preparation of Theonellamide-AMCA.

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Ho C.H., Magtanong L., Barker S.L., Gresham D., Nishimura S., Natarajan P., Koh J.L., Porter J., Gray C.A., Andersen R.J., Giaever G., Nislow C., Andrews B., Botstein D., Graham T.R., Yoshida M, & Boone C. (2009). A molecular barcoded yeast ORF library enables mode-of-action analysis of bioactive compounds. Nature biotechnology, 27(4), 10.1038/nbt.1534.

Publication 2009

Amca Clone Essential genes Growth media Plasmids Strains

Corresponding Organization :

Other organizations : University of Toronto, Princeton University, Kyoto Pharmaceutical University, Kyoto University, Vanderbilt University, University of British Columbia, RIKEN Advanced Science Institute

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Protocol cited in 16 other protocols

Variable analysis

independent variables

Preparation method of plasmid pool
MoBY-ORF clone construction methods
Procedures for sequencing of the MoBY-ORF collection
Procedures for functional complementation analysis of essential genes with MoBY-ORF plasmids
Preparation of Theonellamide-AMCA

dependent variables

Functional complementation analysis of essential genes with MoBY-ORF plasmids

control variables

Strains used in the study
Growth media

controls

Positive controls: Not explicitly mentioned.
Negative controls: Not explicitly mentioned.

Annotations

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