Time-lapse Imaging of A. fumigatus

Well plates were incubated and imaged for 18 h at 37 °C in the IncuCyte ZOOM HD/2CLR time lapse microscopy system (Sartorius) equipped with an IncuCyte ZOOM 10 x PLAN FLUOR objective (Sartorius) as previously described [11 (link)]. Phase images were acquired hourly for all experiments. For live cell co-culture using AF293-GFP, green fluorescence images were obtained hourly with an acquisition time of 400 milliseconds. NT image processing algorithms were applied using our published parameters for A. fumigatus and AF293-GFP [11 (link)].

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Wurster S., Sass G., Albert N.D., Nazik H., Déziel E., Stevens D.A, & Kontoyiannis D.P. (2020). Live imaging and quantitative analysis of Aspergillus fumigatus growth and morphology during inter-microbial interaction with Pseudomonas aeruginosa. Virulence, 11(1), 1329-1336.

Publication 2020

IncuCyte ZOOM HD/2CLR time lapse microscopy IncuCyte ZOOM 10 x PLAN FLUOR objective

Cell culture Fluorescence Microscopy

Corresponding Organization :

Other organizations : The University of Texas MD Anderson Cancer Center, California Institute for Medical Research, Institut National de la Recherche Scientifique, Stanford University

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Variable analysis

independent variables

Incubation time (18 h)
Temperature (37 °C)

dependent variables

Phase images (acquired hourly)
Green fluorescence images (acquired hourly with 400 milliseconds acquisition time)

control variables

IncuCyte ZOOM HD/2CLR time lapse microscopy system (Sartorius)
IncuCyte ZOOM 10 x PLAN FLUOR objective (Sartorius)
NT image processing algorithms

controls

No positive or negative controls were explicitly mentioned.

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