Western Blot Protein Expression Analysis

Western blot analysis was used to detect protein expression and was performed as described previously 15 (link). Antibody used in this study were as follows: p53 (1:2000, cat. sc-126, Santa Cruz, CA, US); p73 (1:1000, cat. 1636-1, Epitomics, US); GRP78 (1:1000, cat. 3183, Cell Signaling Technology (CST), Shanghai, China); PERK (1:1000, cat. 5683, CST); p-PERK (1:1000, cat. 340846, ZEN-BIOSCIENCE, China); ATF6α (1:1000, ab122897, Abcam, Shanghai, China), IRE1α (1:1000, cat. 3294, CST); ATF4 (1:1000, cat. 11815, CST); GAPDH (1:3000, cat. 301341, ZEN-BIOSCIENCE, China); Bim (1:1000, cat. 2933, CST); Caspase 3 (1:1000, cat. 9665, CST).