Assessing 786-O Cell Behavior

The Colony Formation, Transwell Migration, and Invasion assay of 786-O cell were performed as described previously (13 (link)). A minimum of five random fields of view were immediately captured on the 786-O cell. For western blot assay, 786-O cell were incubated with anti-DUSP1 (Cat# AF5286, Affinity Biosciences), anti-β-tubulin (Cat# T0023, Affinity Biosciences), anti-p21 (Cat# AF6290, Affinity Biosciences) and anti-p53 (Cat# AF0879, Affinity Biosciences), following are the specific methods shown (14 (link)).

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Gong Q., Jiang Y., Xiong J., Liu F, & Guan J. (2023). Integrating scRNA and bulk-RNA sequencing develops a cell senescence signature for analyzing tumor heterogeneity in clear cell renal cell carcinoma. Frontiers in Immunology, 14, 1199002.

Publication 2023

anti-β-tubulin anti-p21 anti-p53

Assay Cell Dusp1 Tubulin Western blot assay

Corresponding Organization :

Other organizations : Zhengzhou Children's Hospital, Zhengzhou University, Affiliated Hospital of Youjiang Medical University for Nationalities

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Variable analysis

independent variables

DUSP1 protein expression
β-tubulin protein expression
P21 protein expression
P53 protein expression

dependent variables

Colony formation
Transwell migration
Invasion

control variables

786-O cell line

controls

Positive control: Not specified
Negative control: Not specified

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