Serum Biomarkers Analysis Protocol

Serum glucose level was analyzed using glucose oxidase method (Applygen, Beijing, China) (Wang C. et al., 2016 (link)). Serum insulin level was determined by ELISA using an ultra-sensitive mouse insulin kit (Crystal Chem, USA) (Ding et al., 2016 (link)). Serum levels of total cholesterol, triglycerides, LDL-C, HDL-C, and free fatty acids (FFA) levels were assayed using the calorimetric kits from Nanjing Jiancheng Bioengineering Institute (Nanjing, China) (Jiang et al., 2016 (link); Wang et al., 2019 (link); Liu et al., 2020 (link)). Liver TG was analyzed using Tissue triglyceride assay kit (Applygen, Beijing, China) (Wang C. et al., 2016 (link)).

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Guo F., Xu S., Zhu Y., Zheng X., Lu Y., Tu J., He Y., Jin L, & Li Y. (2020). PPARγ Transcription Deficiency Exacerbates High-Fat Diet-Induced Adipocyte Hypertrophy and Insulin Resistance in Mice. Frontiers in Pharmacology, 11, 1285.

Publication 2020

glucose oxidase method ultra-sensitive mouse insulin kit Tissue triglyceride assay kit

Assay Calorimetric Cholesterol Elisa Free fatty acids Glucose Glucose oxidase Insulin Liver Mouse Serum Tissue Triglyceride

Corresponding Organization : City Of Hope National Medical Center

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Variable analysis

independent variables

Not explicitly mentioned

dependent variables

Serum glucose level
Serum insulin level
Serum total cholesterol
Serum triglycerides
Serum LDL-C
Serum HDL-C
Serum free fatty acids (FFA) levels
Liver triglyceride (TG) level

control variables

Not explicitly mentioned

positive controls

Not specified

negative controls

Not specified

Annotations

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