RAW 264.7 cells were treated with a series of I6CA concentrations or LPS (Sigma-Aldrich Chemical Co). After 24 h of incubation, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetra-zolium bromide (MTT) assay was performed as previously described (Hasnn et al. 2019 (link)). The LPS-treated cells were considered a positive control for activating the macrophages. The morphologic changes were observed under a phase-contrast microscope (Carl Zeiss, Oberkochen, Germany).
Park C., HwangBo H., Lee H., Kim G.Y., Cha H.J., Choi S.H., Kim S., Kim H.S., Yun S.J., Kim W.J., Jeon Y.J, & Choi Y.H. (2020). The immunostimulatory effect of indole-6-carboxaldehyde isolated from Sargassum thunbergii (Mertens) Kuntze in RAW 264.7 macrophages. Animal Cells and Systems, 24(4), 233-241.
Assay BromideCells Macrophages Microscope Phase contrast Raw 264 7 cells
Corresponding Organization :
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Dong-Eui University, Jeju National University, Kosin University, Korea Lift College, Pusan National University, Chungbuk National University
Positive control: LPS-treated cells for activating the macrophages
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