Vaginal Tissue Evaluation for Estrus Cycle Staging

Vaginal tissue was excised from female mice at the point of culling. Following the Methacarn fixation and AB + PAS staining, vaginal tissue allowed assessment of estrus cycle stage^{18 (link)} (Suppl. Fig. 2). Briefly, the presence of mucin containing cells within the epithelium indicated proestrus phase (Suppl. Fig. 2A), shedding of the stratum corneum indicated estrus phase (Suppl. Fig. 2B), leukocyte infiltration indicated metestrus phase (Suppl. Fig. 2C), and a thin stratum corneum without mucin producing cells indicated the diestrus phase (Suppl. Fig. 2D).

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Bennett A.R., Mair I., Muir A., Smith H., Logunova L., Wolfenden A., Fenn J., Lowe A.E., Bradley J.E., Else K.J, & Thornton D.J. (2024). Sex drives colonic mucin sialylation in wild mice. Scientific Reports, 14, 6954.

Publication 2024

Corresponding Organization : University of Manchester

Other organizations : University of Nottingham

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Variable analysis

independent variables

Estrus cycle stage

dependent variables

Presence of mucin containing cells within the epithelium
Shedding of the stratum corneum
Leukocyte infiltration
Thin stratum corneum without mucin producing cells

control variables

Not explicitly mentioned

controls

Positive control: Not mentioned
Negative control: Not mentioned

Annotations

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