Paraffin-embedded hearts were sliced into 5-μm thick sections and used for subsequent histological analyses. Hematoxylin and eosin (H&E) and picrosirius red staining of paraffin-embedded heart sections were visualized using a Leica DMLA microscope (Leica Microsystems, Wetzlar, Germany) equipped with a Retiga 1300i FAST 1394 CCD camera (OImaging, Surrey, BC, Canada) as described previously [2 (link)]. Representative images from each sample are shown. For wheat-germ-agglutinin (WGA) staining, sections were treated with WGA conjugated with Alexa Fluor 488 (Thermo Fisher Scientific, Mississauga, ON, Canada); then, nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) using ProLong Gold Antifade Mountant with DAPI (Thermo Fisher Scientific). The cross-sectional area of cardiomyocytes was quantified using imagej software (National Institutes of Health, Bethesda, MD, USA) as described previously [16 (link)].
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Histological Analysis of Cardiac Tissue
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Alexa fluor 488
Cardiomyocytes
Embedded paraffin
Eosin
Gold
Heart
Hematoxylin
Microscope
Nuclei
Paraffin
Wheat germ agglutinin
Corresponding Organization : University of Alberta
Other organizations : University of Minnesota
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Variable analysis
independent variables
- Paraffin-embedded heart slices with 5-μm thickness
- Histological analyses of paraffin-embedded heart sections using H&E and picrosirius red staining
- Cross-sectional area of cardiomyocytes quantified using ImageJ software
- Paraffin-embedding of heart samples
- Microscopy setup with Leica DMLA microscope and Retiga 1300i FAST 1394 CCD camera
- No positive or negative controls were explicitly mentioned in the description.
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