Synthesis and Modification of RNA Oligonucleotides

2’-O-Me and 2’-F phosphoramidites, universal synthesis columns (MM1-2500-1), and all ancillary RNA synthesis reagents were purchased from Bioautomation (Irving, TX). Symmetrical branching 2’-cyanoethyl, diisopropyl (CED) phosphoramidite (CLP-5215) was obtained from ChemGenes (Wilmington, MA). Cyanine 5 (Cy5) phosphoramidite (10-5915), stearyl phosphoramidite (10-1979), biotin triethyleneglycolyl (TEG) phosphoramidite (10-1955), hexaethyleneglycol phosphoramidite (10-1918), TEG cholesterol phosphoramidite (10-1976), 5’-Amino-Modifier 5 (10-1905), and desalting columns (60-5010) were all purchased from Glen Research (Sterling, VA). PE (phycoerythrin) anti-mouse CD19 antibody (Catalog No. 115508) and APC (allophycocyanin) anti-mouse/human CD45R/B220 antibody (Catalog No. 103212) were acquired from BioLegend (San Diego, CA). MIK665 was from Selleck Chem (Houston, TX). All other reagents were purchased from Sigma-Aldrich unless otherwise specified.