hARF6 ORF cDNA lentiviral clone was obtained from GeneCopoeia (EX-OL00097-LX304-B).
DSS was from Cayman Chemical. Puromycin, hexadimethrine bromide (polybrene), Lucifer Yellow CH dilithium salt, and fluorescein isothiocyanate–dextran were purchased from Sigma. EZ-Link Sulfo-NHS-Biotin and Collagenase, Type I, were obtained from Thermo Fisher Scientific. Polyethylenimine (PEI) was from Polysciences. AGK2 was from MCE. TM, TM-P4-Thal, and IMP-1088 were synthesized as previously described (29 (link), 38 (link), 39 (link)).
Antibodies were obtained from the following sources: Cell Signaling Technology—rabbit anti-E-cadherin (24E10), E-cadherin (24E10) rabbit mAb (Alexa Fluor® 488 Conjugate), rabbit anti-SirT2 (D4050), rabbit anti-HSP90 (C45G5), rabbit anti-Arf6 (D12G6), anti-mouse IgG, HRP-linked antibody (#7076), anti-rabbit IgG, and HRP-linked antibody (#7074); Santa Cruz—anti-β-actin antibody (C4) and anti-GAPDH (0411).
DSS was from Cayman Chemical. Puromycin, hexadimethrine bromide (polybrene), Lucifer Yellow CH dilithium salt, and fluorescein isothiocyanate–dextran were purchased from Sigma. EZ-Link Sulfo-NHS-Biotin and Collagenase, Type I, were obtained from Thermo Fisher Scientific. Polyethylenimine (PEI) was from Polysciences. AGK2 was from MCE. TM, TM-P4-Thal, and IMP-1088 were synthesized as previously described (29 (link), 38 (link), 39 (link)).
Antibodies were obtained from the following sources: Cell Signaling Technology—rabbit anti-E-cadherin (24E10), E-cadherin (24E10) rabbit mAb (Alexa Fluor® 488 Conjugate), rabbit anti-SirT2 (D4050), rabbit anti-HSP90 (C45G5), rabbit anti-Arf6 (D12G6), anti-mouse IgG, HRP-linked antibody (#7076), anti-rabbit IgG, and HRP-linked antibody (#7074); Santa Cruz—anti-β-actin antibody (C4) and anti-GAPDH (0411).
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