Cardiomyocyte Differentiation and Purification

Matrigel (Corning Life Science, Tewksbury, MA, USA) coated 6-well plates were seeded with hiPSCs at a density of 1×10⁵ cells / well and cultured in PSCeasy medium (Cellapy, Beijing, China) at 37°C and 5% CO₂. hiPSCs were differentiated into cardiomyocytes using the small molecule-based method as previously described [51 (link)]. Myocardial purification is performed by the lactate metabolism-selection method at day 10 post myocardial differentiation. And the cells were reseeded on new Matrigel-coated culture plates. Here, the first day of myocardial differentiation was defined as Day 1.

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Zhang J., Peng Y., Fu W., Wang R., Cao J., Li S., Tian X., Li Z., Hua C., Zhai Y., Liu Y., Liu M., Sun J., Li X., Zhao X, & Dong J. (2024). PLEKHM2 deficiency induces impaired mitochondrial clearance and elevated ROS levels in human iPSC-derived cardiomyocytes. Cell Death Discovery, 10, 142.

Publication 2024

Matrigel PSCeasy medium

Corresponding Organization :

Other organizations : First Affiliated Hospital of Zhengzhou University

Top 5 similar protocols

Variable analysis

independent variables

Small molecule-based method for cardiomyocyte differentiation

dependent variables

Cardiomyocyte differentiation efficiency
Myocardial purification by lactate metabolism-selection method

control variables

Matrigel-coated 6-well plates
Seeding density of 1×10^5 hiPSCs per well
Culture in PSCeasy medium at 37°C and 5% CO2

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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