For the retinal ganglion cell-superior colliculus experiment, intravitreal virus injection was performed on P0 C57BL/6 mice with Nanoject II (Drummond) under cold anesthesia. 100 nL of rAAV2/8-Synapsin-Chronos-GFP (titer 1.4×1013 particles/mL) was injected into the eye. AAV particles were produced by the University of North Carolina Chapel Hill Vector Core.
Optogenetic Manipulation of Neural Circuits
For the retinal ganglion cell-superior colliculus experiment, intravitreal virus injection was performed on P0 C57BL/6 mice with Nanoject II (Drummond) under cold anesthesia. 100 nL of rAAV2/8-Synapsin-Chronos-GFP (titer 1.4×1013 particles/mL) was injected into the eye. AAV particles were produced by the University of North Carolina Chapel Hill Vector Core.
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Corresponding Organization :
Other organizations : Massachusetts Institute of Technology, Janelia Research Campus, Howard Hughes Medical Institute, IIT@MIT, University of Alberta, BGI Group (China), University of Pennsylvania, University of Cologne, McGovern Institute for Brain Research
Protocol cited in 38 other protocols
Variable analysis
- Plasmid (pCAG-opsin-GFP, pCAG-FLEX-rc[Chronos-GFP], pCAG-FLEX-Chrimson-mOrange2, pCAG-Chrimson-tdTomato, pCAG-Cre) used for electroporation
- Viral vector (rAAV2/8-Synapsin-Chronos-GFP) used for intravitreal injection
- Outcomes of electroporation and virus injection experiments, such as neuronal expression of opsin proteins and fluorescent reporters
- Mouse strain (C57BL/6J and C57BL/6)
- Timing of procedures (E16-timed pregnant mice, P0 mice)
- Anesthesia (ketamine-xylazine) and analgesia (buprenorphine)
- Electroporation parameters (5 square pulses, 50ms width, 1Hz, 35V)
- Viral vector titer (1.4×10^13 particles/mL)
- Not explicitly mentioned
- Not explicitly mentioned
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