BDH2 Overexpression in Cell Lines

We used the following antibodies: BDH2 (1:1000, HPA004428, Sigma-Aldrich, St. Louis, MO, USA), β-catenin (1:1000, sc-376841, Santa Cruz Biotechnology, Santa Cruz, CA, USA), E-cadherin (1:1000, #3195P) and vimentin (VIM; 1:1000, #5741P, both Cell Signaling Technology, Beverly, USA), secreted protein acidic and cysteine rich (SPARC; 1:1000, #66426-1, SANYING, Wuhan, China) and GAPDH (1:10000, #5174P, Cell Signaling Technology). The secondary antibody 680RD goat anti-mouse and IRDye 800CW goat anti-rabbit antibodies were from LI-COR Biosciences (Lincoln, NE, USA).
BDH2 plasmid was purchased from Origene (Rockville, MD, USA); the open-reading frame of BDH2 was subcloned into the pCMV6-Entry vector. The protocol of transfection has been described.^{20 (link)}