The strains used in this study were selected from each group based on our previous results [9 (link)]. The two main selection criteria are the capacity of the strain to induce platelet activation and the profile toward the platelet inhibitory effect (Table 1 ).
The strains represent the following profiles: E. coli J53, platelet sensitive strain which induces platelet activation; E. coli K12, platelet resistant strain which induces platelet activation; and E. coli LH30, platelet resistant strain which does not induce platelet activation (Table 1 ).
Identification was confirmed using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry and the Biotyper database (Bruker, Dresden, Germany). Strains were grown at 37 °C in an overnight culture of Columbia agar +5% sheep blood (bioMérieux, Marcy l’Etoile, France). After 18 h of incubation at 37 °C, the colonies were removed and suspended in 0.9% NaCl medium to obtain the required concentrations: 1 × 108 CFU (colony format units).
The strains represent the following profiles: E. coli J53, platelet sensitive strain which induces platelet activation; E. coli K12, platelet resistant strain which induces platelet activation; and E. coli LH30, platelet resistant strain which does not induce platelet activation (
Identification was confirmed using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry and the Biotyper database (Bruker, Dresden, Germany). Strains were grown at 37 °C in an overnight culture of Columbia agar +5% sheep blood (bioMérieux, Marcy l’Etoile, France). After 18 h of incubation at 37 °C, the colonies were removed and suspended in 0.9% NaCl medium to obtain the required concentrations: 1 × 108 CFU (colony format units).
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