Brain MRI and PET Imaging in Animals

First, a T1-weighted brain MRI scan of each subject was acquired (Signa Excite HDTx 3.0 T scanner, GE Healthcare). Afterward, animals underwent a 60-min transmission scan using a rotating ⁶⁸Ge/⁶⁸Ga rod source followed by a 91-min dynamic emission PET scan (SHR-38000, Hamamatsu Photonics) with arterial blood sampling. Animals were injected with (R)-[¹¹C]verapamil (954.2 ± 45.6 MBq, with 99.3 ± 0.2% radiochemical purity and a molar activity (MA) higher than 20 GBq/μmol), or [¹⁸F]MC225 (684 ± 64 MBq, with a purity of 97.6 ± 1.0% and a MA higher than 36 GBq/μmol), as a single bolus (over 30 s) via the saphenous vein, at the start of the emission scan (for more information [35 , 36 (link)]).

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García-Varela L., Vállez García D., Aguiar P., Kakiuchi T., Ohba H., Harada N., Nishiyama S., Tago T., Elsinga P.H., Tsukada H., Colabufo N.A., Dierckx R.A., van Waarde A., Toyohara J., Boellaard R, & Luurtsema G. (2021). Head-to-head comparison of (R)-[11C]verapamil and [18F]MC225 in non-human primates, tracers for measuring P-glycoprotein function. European Journal of Nuclear Medicine and Molecular Imaging, 48(13), 4307-4317.

Publication 2021

SHR-38000

Animals Arterial Brain Molar Mri scan Pet scan Radiochemical Saphenous vein Scan Transmission Verapamil

Corresponding Organization :

Other organizations : University Medical Center Groningen, University of Groningen, Hamamatsu Photonics (Japan), Tokyo Metropolitan Institute of Gerontology, University of Bari Aldo Moro

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Variable analysis

independent variables

Injected radiotracer: (R)-[11C]verapamil or [18F]MC225

dependent variables

Time-dependent uptake and distribution of the injected radiotracer measured using dynamic emission PET scan

control variables

T1-weighted brain MRI scan of each subject acquired using a Signa Excite HDTx 3.0 T scanner (GE Healthcare)
60-min transmission scan using a rotating 68Ge/68Ga rod source
91-min dynamic emission PET scan (SHR-38000, Hamamatsu Photonics)
Arterial blood sampling
Injection of radiotracer as a single bolus (over 30 s) via the saphenous vein, at the start of the emission scan

controls

Positive control: No information provided.
Negative control: No information provided.

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