Adoptive Transfer of OT-I Transgenic T Cells

C57BL/6 mice were from Charles River/NCI. Nur77-GFP reporter (C57BL/6-Tg(Nr4a1-EGFP/cre)820^Khog/J) (37 (link)), OT-I transgenic (C57BL/6-Tg(TcraTcrb)1100^Mjb/J) (38 (link)), Thy1.1 congenic mice (B6.PL-Thy1^a/CyJ) (39 (link)), Rag1^ko mice (B6.Rag1^em10Lutzy) and Perforin^ko (C57BL/6-Prf1tm1Sdz/J) (40 (link)) mice, all from Jackson Laboratories, were bred and maintained in a pathogen-free facility at the University of Virginia. Six to 12-week-old Nur77-GFP x (OT-I x Thy1.1) F1 mice were the source of mice expressing OT-I⁺Thy1.1⁺Nur77-GFP⁺ cells used for adoptive transfer. All procedures were approved by the University of Virginia Animal Care and Use Committee in accordance with the NIH Guide for Care and Use of Laboratory Animals.