Antiviral Activity of Selenium Nanoparticles

Na₂SeO₃, vitamin C, chitosan, propidium iodide, and 6-coumarin were purchased
from Sigma. Dulbecco’s modified eagle medium (DMEM) and fetal
bovine serum (FBS) were gained from Gibco. Thiazolyl blue tetrazolium
bromide (MTT) and lyso tracker were used from Sigma in the study.
JNK, p53, caspase-3, BAD, JAK, p-STAT3, Akt, and β-actin monoclonal
antibodies were obtained from Cell Signaling Technology (CST). Madin-Darby
canine kidney cells (MDCK) were obtained from American Type Culture
Collection (ATCC CCL-34). H3N2 influenza virus was provided by Virus
laboratory, Guangzhou Institute of Pediatrics, Guangzhou Women and
Children’s Medical Center, Guangzhou Medical University.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Xu T., Lai J., Su J., Chen D., Zhao M., Li Y, & Zhu B. (2023). Inhibition of H3N2 Influenza Virus Induced Apoptosis by Selenium Nanoparticles with Chitosan through ROS-Mediated Signaling Pathways. ACS Omega, 8(9), 8473-8480.

Publication 2023

Na2SeO3 vitamin C chitosan propidium iodide 6-coumarin Dulbecco’s modified eagle medium (DMEM) fetalbovine serum (FBS) Thiazolyl blue tetrazoliumbromide (MTT) lyso tracker caspase-3 p-STAT3 Madin-Darbycanine kidney cells (MDCK) ATCC CCL-34

Actin Caspase 3 Ccl 34 Cells Chitosan Coumarin Eagle H3n2 virus Influenza Influenza virus Kidney Lyso tracker Propidium iodide Serum Stat3 Thiazolyl blue Vitamin c Women

Corresponding Organization : Guangzhou Medical University

Top 5 similar protocols

Variable analysis

independent variables

Na₂SeO₃
Vitamin C
Chitosan
Propidium iodide
6-coumarin
H3N2 influenza virus

dependent variables

Cell viability (measured by MTT assay)
Lysosome activity (measured by lyso tracker)

control variables

Dulbecco's modified eagle medium (DMEM)
Fetal bovine serum (FBS)
Madin-Darby canine kidney cells (MDCK)
JNK, p53, caspase-3, BAD, JAK, p-STAT3, Akt, and β-actin monoclonal antibodies

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!