The human cervical carcinoma cell line HeLa and human embryonic kidney cell line HEK293A were obtained from ATCC (Manassas, VA, USA). LATS1/2-DKO HEK293A cells were generously provided by Dr Kun-Liang Guan, the Department of Pharmacology and Moores Cancer Center at University of California San Diego (43 (link)). The cells were maintained in Dulbecco's modified Eagle medium (DMEM; WELGENE, Gyeongsan, Korea) supplemented with 10% fetal bovine serum (FBS; Gibco, Gaithersburg, MD, USA), 100 U/ml penicillin, and 100 μg/ml streptomycin (WELGENE). The cells were incubated at 37°C in a humidified atmosphere containing 5% CO2.
Protocol detail
Cell Culture Protocol for HeLa and HEK293A
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Atmosphere
Cancer
Carcinoma
Cell line
Cells
Cervical
Eagle
Embryonic
Hela
Human
Kidney
Lats1
Penicillin
Streptomycin
Corresponding Organization : Korea Institute of Toxicology
Other organizations : Chonnam National University, Seoul National University Hospital
Top 5 similar protocols
Variable analysis
independent variables
- LATS1/2-DKO (double knockout) of the LATS1 and LATS2 genes in HEK293A cell line
- Not explicitly mentioned
- HeLa (human cervical carcinoma) cell line
- HEK293A (human embryonic kidney) cell line
- Cell culture conditions: Dulbecco's modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 100 U/ml penicillin, and 100 μg/ml streptomycin, incubated at 37°C in a humidified atmosphere containing 5% CO2
- Positive control: HEK293A (human embryonic kidney) cell line
- Negative control: not explicitly mentioned
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!