Pharmacological Modulation of Murine Behaviors

SNAP: Female mice received an s.c. injection with 8 mg/kg SNAP (Sigma-Aldrich; N3398) diluted in 100 µL saline 15 min before behavioral testing, and the chosen dose was based on previous reports (24 (link)). Kisspeptin: Female mice received an s.c. injection with 0.52 µg/kg kisspeptin-10 (Kiss-10; rodent metastin [45 (link)–54 (link, link, link, link, link, link, no link found, link, no link found)] amide; GeneCust YY-10-NH2) diluted in 100 µL saline 30 min before the behavioral test based on previous reports (24 (link)). sildenafil citrate: Female mice received an i.p. injection with 15 mg/kg sildenafil citrate (Sigma-Aldrich; 171599–83-0) 1 h before behavioral assessment based on previous trials from our laboratory. sildenafil citrate was first diluted in DMSO to make up a stock solution of 25 mg/mL and further diluted with sterile saline to make up a working solution of 15 mg/mL for s.c. injection on the day of the injection.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Silva M.S., Decoster L., Trova S., Mimouni N.E., Delli V., Chachlaki K., Yu Q., Boehm U., Prevot V, & Giacobini P. (2022). Female sexual behavior is disrupted in a preclinical mouse model of PCOS via an attenuated hypothalamic nitric oxide pathway. Proceedings of the National Academy of Sciences of the United States of America, 119(30), e2203503119.

Publication 2022

sildenafil citrate

Amide Behavioral test Dmso Female Kisspeptin Kisspeptin 10 Metastin Mg citrate Mice Rodent Saline Sildenafil Sildenafil citrate Sterile

Corresponding Organization :

Other organizations : Université de Lille, Inserm, Centre Hospitalier Universitaire de Lille, Saarland University

Top 5 similar protocols

Variable analysis

independent variables

SNAP (Sigma-Aldrich; N3398) at 8 mg/kg, administered subcutaneously 15 min before behavioral testing
Kisspeptin-10 (Kiss-10; rodent metastin [45-54] amide; GeneCust YY-10-NH2) at 0.52 µg/kg, administered subcutaneously 30 min before behavioral testing
Sildenafil citrate (Sigma-Aldrich; 171599-83-0) at 15 mg/kg, administered intraperitoneally 1 h before behavioral assessment

dependent variables

Behavioral outcomes measured during the testing sessions

control variables

Female mice used as the subjects
Saline used as the vehicle for diluting the drugs

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!