HPLC Analysis of Free Amino Acids

High performance liquid chromatography measurements of free amino acids were performed on a 1260 Infinity HPLC system equipped with a fluorescence detector (Agilent Technologies, Waldbronn, Germany) and a Poroshell HPH-C18 separation column (4.6 mm × 100 mm, particle size 2.7 mm; Agilent Technologies). Before measuring, ammonium was precipitated from the samples by a 1:1 dilution with sodium tetraphenylborate (250 mM). After vigorously mixing and centrifugation (5 min, 13,000 rpm, 4°C), the ammonium-free samples were transferred in HPLC vials. The HPLC method was used as described previously (Trautwein et al., 2016 (link); Dannheim et al., 2017b (link)) with the minor modification that mobile phase A was changed to 25 mM sodium acetate (pH 6.5).

Free full text: Click here

Hofmann J.D., Otto A., Berges M., Biedendieck R., Michel A.M., Becher D., Jahn D, & Neumann-Schaal M. (2018). Metabolic Reprogramming of Clostridioides difficile During the Stationary Phase With the Induction of Toxin Production. Frontiers in Microbiology, 9, 1970.

Publication 2018

1260 Infinity HPLC system Poroshell HPH-C18 separation column

Acids Ammonium Centrifugation Dilution Fluorescence Hplc Sodium acetate Sodium tetraphenylborate

Corresponding Organization : Leibniz Institute DSMZ – German Collection of Microorganisms and Cell Cultures

Other organizations : Universität Greifswald

Top 5 similar protocols

Variable analysis

independent variables

None explicitly mentioned

dependent variables

Concentrations of free amino acids

control variables

1260 Infinity HPLC system equipped with a fluorescence detector
Poroshell HPH-C18 separation column (4.6 mm × 100 mm, particle size 2.7 mm)
Sodium tetraphenylborate (250 mM) used to precipitate ammonium from samples
Centrifugation (5 min, 13,000 rpm, 4°C) to obtain ammonium-free samples
HPLC method used as described previously (Trautwein et al., 2016; Dannheim et al., 2017b) with the minor modification that mobile phase A was changed to 25 mM sodium acetate (pH 6.5)

controls

None explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!