M. smegmatis cells were grown in standard Middlebrook 7H9 medium (BD/Difco) containing 0.085% NaCl, 0.5% albumin, 0.2% glucose, 0.5% glycerol, and 0.05% Tween-80 at 37 °C with shaking at 200 rpm [35 (link)]. Optical density of the cells (absorbance at 600 nm) was measured to determine the growth of the cells via a spectrophotometer (Thermo Scientific biomate 5, Waltham, MA, USA).
Free full text: Click here