Growth Kinetics of M. smegmatis

M. smegmatis cells were grown in standard Middlebrook 7H9 medium (BD/Difco) containing 0.085% NaCl, 0.5% albumin, 0.2% glucose, 0.5% glycerol, and 0.05% Tween-80 at 37 °C with shaking at 200 rpm [35 (link)]. Optical density of the cells (absorbance at 600 nm) was measured to determine the growth of the cells via a spectrophotometer (Thermo Scientific biomate 5, Waltham, MA, USA).

Free full text: Click here

, & Elitas M. (2018). On-Chip Isoniazid Exposure of Mycobacterium smegmatis Penicillin-Binding Protein (PBP) Mutant Using Time-Lapse Fluorescent Microscopy. Micromachines, 9(11), 561.

Publication 2018

biomate 5

Albumin Glucose Glycerol M cells Nacl Optical Tween 80

Top 5 similar protocols

Variable analysis

independent variables

Growth medium composition (Middlebrook 7H9 medium with 0.085% NaCl, 0.5% albumin, 0.2% glucose, 0.5% glycerol, and 0.05% Tween-80)
Temperature (37 °C)
Shaking speed (200 rpm)

dependent variables

Growth of M. smegmatis cells (measured by optical density at 600 nm)

control variables

M. smegmatis cells

controls

No positive or negative controls were explicitly mentioned.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!