Focal Cerebral Ischemia in Genetic Mice

Adult WNK3 WT (female WNK^+/+ and male WNK^Y/+), adult WNK3 KO (female WNK3^-/- and male WNK3^Y/-), and adult male NKCC1 WT or KO mice (NKCC1^+/+ and NKCC1^-/- mice, originally developed by Flagella et al., ^{25 (link)}, each weighing approximately 25–30 g at the ages of 2-3 months, were used in this study. Focal cerebral ischemia was induced by 60-min middle cerebral artery (MCA) occlusion, as previously described ^{26 (link)} and detailed description is provided in the online-only Data Supplement.

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Begum G., Yuan H., Kahle K.T., Li L., Wang S., Shi Y., Shmukler B.E., Yang S.S., Lin S.H., Alper S.L, & Sun D. (2015). Inhibition of WNK3 kinase signaling reduces brain damage and accelerates neurological recovery after stroke. Stroke; a journal of cerebral circulation, 46(7), 1956-1965.

Publication 2015

Adult Adult female Cerebral ischemia Female Flagella Male Mice Middle cerebral artery occlusion Supplement

Corresponding Organization :

Other organizations : Harvard University, Boston Children's Hospital, National Defense Medical Center, Tri-Service General Hospital, Geriatric Research Education and Clinical Center, Beth Israel Deaconess Medical Center, University of Pittsburgh

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Protocol cited in 9 other protocols

Variable analysis

independent variables

Genotype (WT vs. KO) of WNK3 in adult mice
Genotype (WT vs. KO) of NKCC1 in adult male mice

dependent variables

Focal cerebral ischemia induced by 60-min middle cerebral artery (MCA) occlusion

control variables

Age (2-3 months) of all mice
Weight (approximately 25-30 g) of all mice

controls

Positive control: Adult male NKCC1 WT mice (NKCC1+/+)
Negative control: Adult male NKCC1 KO mice (NKCC1-/-)

Annotations

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