Detecting DNA Adducts and TP53 Mutations

DNA was isolated from the renal cortex and tumor tissues by standard phenol-chloroform extraction techniques. The level of AL-DNA adducts in the renal cortex DNA (10–20 µg) was determined using ³²P-postlabeling polyacrylamide gel electrophoresis, as previously described (13 (link)). The TP53-specific mutations were identified using the AmpliChip p53 Research Test (Roche Molecular Diagnostics, Pleasanton, CA), sensitively detecting all single base-pair substitutions and single-base deletions (13 (link)).

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Castells X., Karanović S., Ardin M., Tomić K., Xylinas E., Durand G., Villar S., Forey N., Le Calvez-Kelm F., Voegele C., Karlović K., Mišić M., Dittrich D., Dolgalev I., McKay J., Shariat S.F., Sidorenko V.S., Fernandes A., Heguy A., Dickman K.G., Olivier M., Grollman A.P., Jelaković B, & Zavadil J. (2015). Low-coverage exome sequencing screen in formalin-fixed paraffin-embedded tumors reveals evidence of exposure to carcinogenic aristolochic acid. Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, 24(12), 1873-1881.

Publication 2015

AmpliChip p53 Research Test

Base pair Chloroform Deletions Dna adducts Molecular diagnostics Mutations Phenol Polyacrylamide gel electrophoresis Renal cortex Tissues Tumor

Corresponding Organization : Centre International de Recherche sur le Cancer

Other organizations : University of Zagreb, College of Slavonski Brod, Cornell University, NYU Langone Health, Stony Brook University

Top 5 similar protocols

Variable analysis

independent variables

Isolation of DNA from renal cortex and tumor tissues

dependent variables

Level of AL-DNA adducts in the renal cortex DNA
TP53-specific mutations

control variables

Standard phenol-chloroform extraction techniques used for DNA isolation

controls

No positive or negative controls are explicitly mentioned in the input protocol.

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!