Quantifying Lipoprotein Secretion in Mice
Corresponding Organization :
Other organizations : New York University, Columbia University Irving Medical Center, University at Albany, State University of New York
Variable analysis
- Intraperitoneal injection of 200 µCi of [35S] methionine/cysteine protein labeling mix
- Intraperitoneal injection of 1,000 mg/kg pluronic F127 poloxamer-407
- Triglyceride secretion
- Total plasma apoB100 and apoB48 secretion
- Overnight fast
- Plasma from Apobec1−/− mice (which express only apo B100) was used as a control to identify apoB100 and apoB48 bands.
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