18F-FDG was supplied by PETNET solutions, Nottingham, UK. Imaging was performed using the Inveon Multimodality ™ PET scanner from Siemens Medical Solutions.
Mice received approximately 15MBq 18F-FDG administered as an i.v. bolus. Following 18F- FDG injection, anaesthesia was maintained for a 45-minute uptake period followed by a 20-minute emission PET scan. Animals were humanely euthanised following scanning. Data were acquired using Inveon Acquisition Workplace (IAW) software (Siemens) version 1.4.3 and analysed using Inveon Reconstruction Workplace (IRW) software (Siemens) version 2.2.0. The performance of the scanner has been documented previously [17 (link)]. Images were reconstructed using the order subset expectation maximisation (OSEM)/maximum a posterior (MAP) algorithm. Regions of interest (ROI’s) were drawn manually using the 3D visualisation package of IRW software. Data were expressed as the maximum standardised uptake value (MaxSUV). MaxSUV was calculated using the formula described by Gambhir et al, where ID is the injected activity [18 ].
Mice received approximately 15MBq 18F-FDG administered as an i.v. bolus. Following 18F- FDG injection, anaesthesia was maintained for a 45-minute uptake period followed by a 20-minute emission PET scan. Animals were humanely euthanised following scanning. Data were acquired using Inveon Acquisition Workplace (IAW) software (Siemens) version 1.4.3 and analysed using Inveon Reconstruction Workplace (IRW) software (Siemens) version 2.2.0. The performance of the scanner has been documented previously [17 (link)]. Images were reconstructed using the order subset expectation maximisation (OSEM)/maximum a posterior (MAP) algorithm. Regions of interest (ROI’s) were drawn manually using the 3D visualisation package of IRW software. Data were expressed as the maximum standardised uptake value (MaxSUV). MaxSUV was calculated using the formula described by Gambhir et al, where ID is the injected activity [18 ].
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