Western Blot Analysis of Apoptosis Markers

For Western blotting analysis, cells were lysed as described previously [19 (link)]. Primary antibody, KIFC1 (H00003833-M01, Abnova, Taipei, Taiwan), Bcl-2 (sc-7382, Santa Cruz Biotechnology, Santa Cruz, CA, USA), Bax (sc-7480, Santa Cruz Biotechnology, Santa Cruz, CA, USA), cleaved PARP (c-PARP) (#5625, Cell Signaling Technology, Inc., Danvers, MA, USA), cleaved caspase-3 (c-caspase-3) (#9661, Cell Signaling Technology, Inc., Danvers, MA, USA) were used. β-Actin (Sigma-Aldrich, St. Louis, MO, USA) was used as a loading control.

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Sekino Y., Oue N., Koike Y., Shigematsu Y., Sakamoto N., Sentani K., Teishima J., Shiota M., Matsubara A, & Yasui W. (2019). KIFC1 Inhibitor CW069 Induces Apoptosis and Reverses Resistance to Docetaxel in Prostate Cancer. Journal of Clinical Medicine, 8(2), 225.

Publication 2019

Bcl-2 Bax cleaved PARP (c-PARP) cleaved caspase-3 (c-caspase-3) β-Actin

Actin Antibody Bcl 2 Caspase 3 Cells Western blotting analysis

Corresponding Organization : Hiroshima University

Other organizations : Kyushu University

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Variable analysis

independent variables

Primary antibody used for Western blotting analysis

dependent variables

Protein expression levels of KIFC1, Bcl-2, Bax, cleaved PARP, and cleaved caspase-3

control variables

Cell lysis procedure (described previously in reference 19)
β-Actin used as a loading control

controls

No positive or negative controls were explicitly mentioned

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