Serum tOC will be analyzed as described previously [9 (link),10 (link),19 (link),39 (link)]. In brief, tOC will be measured using an automated immunoassay (Elecsys 170; Roche Diagnostics). Serum ucOC will be measured by the same immunoassay after absorption of cOC on 5 mg/mL hydroxyl-apatite slurry, following the method described by Gundberg et al [77 (link)].
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