QPI of MCF-7, BT-474, and HeLa cells was performed as described in Yu et al.74 (link). Fluorescence images were obtained with an EM-CCD C9100 camera (Hamamatsu Photonics) and an X-Cite Series 120 Q (Lumen Dynamics) source. Image collection occurred every 5 min for 12 h at 14–16 imaging locations containing cells plated with sufficient spacing to enable automated image processing and biomass segmentation.
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