Vagus Nerve Stimulation in tMCAO Mice

VNS was performed firstly at 30 mins, 1 day, and 2 days after the establishment of tMCAO. Invasive VNS was performed as previously described (28 (link)). Briefly, the mice were anesthetized intraperitoneally with 3.5% chloral hydrate (0.1ml/10g). Bipolar electrodes were wrapped delicately around the right cervical VN and sutured to the sternocleidomastoid muscle. VNS (0.5 mA, 5 Hz) was administered through a Grass Model S48 stimulator (Grass Technologies, USA) for 30 seconds every five minutes for one hour.

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Xie C., Gao X., Liu G., Tang H, & Li C. (2023). USP10 is a potential mediator for vagus nerve stimulation to alleviate neuroinflammation in ischaemic stroke by inhibiting NF-κB signalling pathway. Frontiers in Immunology, 14, 1130697.

Publication 2023

Grass Model S48 stimulator

Cervical Chloral hydrate Grass Mice Muscle Seconds

Corresponding Organization : Second Affiliated Hospital of Chongqing Medical University

Other organizations : Chengdu University, First Affiliated Hospital of Chongqing Medical University

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Variable analysis

independent variables

Timing of VNS (30 mins, 1 day, 2 days after tMCAO)

dependent variables

Not explicitly mentioned

control variables

Anesthesia (3.5% chloral hydrate, 0.1ml/10g)
Electrode placement (bipolar electrodes wrapped around right cervical VN and sutured to sternocleidomastoid muscle)
VNS parameters (0.5 mA, 5 Hz, 30 seconds every five minutes for one hour)

controls

No positive or negative controls were explicitly mentioned.

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