Total RNA was extracted using the Qiagen RNAeasy kit with on-column DNase digestion according to the manufacturer’s instructions. qRT-PCR was performed on a Bio-Rad CFX Connect Real-Time System machine using iTaq Universal SYBR Green 1 Step Reaction Mix (Bio-Rad). Expression was normalized against the RPL19 house keeper gene. PCR conditions and primer sequences were as described previously [3 (link)].
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