A549, H460 and H1299 cells were purchased from ATCC and cultured in RPMI-1640 medium supplemented with 10% FBS and penicillin/streptomycin. For stable clones generation, cells were transfected using SureSilencing shRNA plasmids (Qiagen, Valencia, CA, USA) and cells stably integrating the silencing plasmid were selected and sub-cultured in medium containing 1 mg/ml of puromycin. HeLa-DR13-9 cells were cultured as previously described [50 (link)]. Plasmid and siRNA transfections were performed using Lipofectamine-2000 or Oligofectamine (Invitrogen, Carlsbad, CA, USA), respectively, according to manufacturer's instructions. Control and anti-RanBP9 siRNAs were purchased from Santa-Cruz (Santa Cruz, CA, USA). Plasmid for transient expression of RanBP9-GFP fusion protein, cloned in pCMV6-AC-GFP vector, was purchased from Origene. For irradiation experiments, cells were treated using an X-ray linear accelerator (Gammacell 40 Exactor, Best Theratonics, Ottawa, Canada) with doses ranging from 1 to 10 Gy. For ATM inhibition, cells were treated with 10 μM KU-55933 (EMD-Millipore, Billerica, MA, USA) for 1 h before ATM activation.
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